Genome engineering

Browse key articles nominated by our Editors

A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

from Horticulture Research

A novel system for correcting large-scale chromosomal aberrations: ring chromosome correction via reprogramming into induced pluripotent stem cell (iPSC)

from Chromosoma

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Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures

from Cell Research

 

Am I ready for CRISPR? A user's guide to genetic screens

from Nature Reviews Genetics

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An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design

from Scientific Reports

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An extended model for culture-dependent heterogenous gene expression and proliferation dynamics in mouse embryonic stem cells

from npj Systems Biology and Applications

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Applying CRISPR–Cas9 tools to identify and characterize transcriptional enhancers

from Nature Reviews Molecular Cell Biology

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Beyond editing to writing large genomes

from Nature Reviews Genetics

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Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation

from Nature Reviews Molecular Cell Biology

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Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

from Nature Communications

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Computational correction of copy number effect improves specificity of CRISPR–Cas9 essentiality screens in cancer cells

from Nature Genetics

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CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa

from npj Regenerative Medicine

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CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein

from Molecular Genetics and Genomics

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CRISPR/Cas9-mediated somatic and germline gene correction to restore hemostasis in hemophilia B mice

from Human Genetics

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Design of Amine-Modified Graft Polyesters for Effective Gene Delivery Using DNA-Loaded Nanoparticles

from Pharmaceutical Research

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Experimental test and refutation of a classic case of molecular adaptation in Drosophila melanogaster

from Nature Ecology & Evolution

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Genome edited animals: Learning from GM crops?

from Transgenic Research

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Genome editing for the reproduction and remedy of human diseases in mice

from Journal of Human Genetics

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Genome editing in livestock: Are we ready for a revolution in animal breeding industry?

from Transgenic Research

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How driving endonuclease genes can be used to combat pests and disease vectors

from BMC Biology

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i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases

from Genome Biology

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Improved DNA/Emulsion Complex Stabilized by poly(ethylene glycol) Conjugated Phospholipid

from Pharmaceutical Research

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Intravenous delivery of adeno-associated virus 9-encoded IGF-1Ea propeptide improves post-infarct cardiac remodelling

from npj Regenerative Medicine

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Mesenchymal stem cell-based gene therapy for erectile dysfunction

from International Journal of Impotence Research

Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion

from Current Genetics

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Next-generation mammalian genetics toward organism-level systems biology

from npj Systems Biology and Applications

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Non-viral delivery of genome-editing nucleases for gene therapy

from Gene Therapy

Obstruction of BRAFV600E transcription by complementary PNA oligomers as a means to inhibit BRAF-mutant melanoma growth

from Cancer Gene Therapy

One small edit for humans, one giant edit for humankind? Points and questions to consider for a responsible way forward for gene editing in humans

from European Journal of Human Genetics

One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs

from Cell Research

Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants

from Scientific Reports

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Optimizing targeted gene delivery: Chemical modification of viral vectors and synthesis of artificial virus vector systems

from The AAPS Journal

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Precision genome engineering through adenine and cytosine base editing

from Nature Plants

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Principles and methods of in-silico prioritization of non-coding regulatory variants

from Human Genetics

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Progress and prospects in plant genome editing

from Nature Plants

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Pyramiding of transgenic Pm3 alleles in wheat results in improved powdery mildew resistance in the field

from Theoretical and Applied Genetics

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Radiation-enhanced delivery of plasmid DNA to tumors utilizing a novel PEI polyplex

from Cancer Gene Therapy

Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications

from World Journal of Microbiology and Biotechnology

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Regulation of cancer epigenomes with a histone-binding synthetic transcription factor

from npj Genomic Medicine

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RNA virus interference via CRISPR/Cas13a system in plants

from Genome Biology

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Spending limited resources on de-extinction could lead to net biodiversity loss

from Nature Ecology & Evolution

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Structural insights into the inactivation of CRISPR-Cas systems by diverse anti-CRISPR proteins

from BMC Biology

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Synchronized DNA cycling across a bacterial population

from Nature Genetics

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Synthetic gene regulation for independent external induction of the Saccharomyces cerevisiae pseudohyphal growth phenotype

from Communications Biology

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Synthetic genome recoding: new genetic codes for new features

from Current Genetics

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Systematic design for trait introgression projects

from Theoretical and Applied Genetics

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Targeted in vivo knock-in of human alpha-1-antitrypsin cDNA using adenoviral delivery of CRISPR/Cas9

from Gene Therapy

Targeted recombination between homologous chromosomes for precise breeding in tomato

from Nature Communications

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The application of powerful promoters to enhance gene expression in industrial microorganisms

from World Journal of Microbiology and Biotechnology

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Three-dimensional genome architecture and emerging technologies: looping in disease

from Genome Medicine

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Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology

from Chromosoma

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UDiTaS™, a genome editing detection method for indels and genome rearrangements

from BMC Genomics

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Updated summary of genome editing technology in human cultured cells linked to human genetics studies

from Journal of Human Genetics

 

Recommended book

9781493926879

CRISPR: Methods and Protocols

​​​​​​​Editors: Magnus Lundgren, Emmanuelle Charpentier, Peter Fineran

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