HILIC, SFC and other polar separation techniques

Course combines theory, practice and expert tips

The separation of very polar molecules had its origins many decades ago - despite this, apart from IC, no other techniques have been able to establish themselves in most laboratories. What you can use and how you use the technology is important to know and can be learned!

Springer Campus Certificate Courses

  • Continuing education at top level
  • Different learning formats: From self-study to online learning to Attendance courses
  • Incl. consulting and personal support

Course content and process

Contents Polar Chromatography

  • Apparatus setup of HPLC and SFC
  • Basic terms in IC, HILIC and SFC
  • Basic concepts in further polar chromatography techniques

Content HILIC(-MS)

  • Separation and detection of very polar molecules with HILIC-MS
  • Stationary HILIC phases (for MS coupling)
  • Mobile HILIC phases (for MS coupling)
  • Other necessary conditions for MS coupling

Contents SFC(-MS)

  • Separation and detection of very polar molecules with SFC-MS
  • Stationary SFC phases (for MS coupling)
  • Mobile SFC phases (for MS coupling)
  • Other necessary conditions for MS coupling
  • Content Application of polar chromatography
  • Examples from the fields of bioanalysis, environmental analysis and food analysis
  • Screening approaches for polar molecules
  • Examples of course participants (if known in advance)

Contents Chromatographic couplings

  • LC-LC-MS systems for the simultaneous detection of molecules of different polarity
  • RPLC-HILIC-MS: structure and application
  • 2D- LC-MS: structure and application

Your top speaker:

PD Dr. habil. Thomas Letzel, born in 1970, studied chemistry (1992-1998) at the Technical University of Munich and the LMU Munich and received his doctorate in 2001 with an environmental analysis topic at the Technical University of Munich and then completed a two-year postdoctoral stay at the Vrijen Universiteit Amsterdam. In 2009 he habilitated at the Technical University of Munich, where he has been teaching and researching as a private lecturer ever since. Until 2018 he was head of the analytical research group AFG at the TUM, 2014-2017 acting head of the professorship for pharmaceutical analysis at the LMU and has been the founder and managing director of the Analytical Research Institute for Non-Target Screening GmbH (AFIN-TS) since 2018. To date, he has developed many new analytical platforms, workflows and strategies for the detection, identification and determination of functional properties of organic molecules from complex mixtures in the various scientific disciplines. The results are reflected in his more than 75 scientific publications and 4 books to date.

All information at a glance:

Course format:  

Online course

Cooperation partner: 

Analytisches Forschungsinstitut für Non-Target Screening GmbH

Course duration:

3 days for 3 hours in the afternoon

Target audience:

The course is aimed at beginners and users who want to use the new chromatographic techniques HILIC, SFC and others to analyze very polar molecules and may also want to use mass spectrometric detection. The aim of this manufacturer-independent seminar is to impart basic knowledge of polar chromatography and to make it easier to get started with this technique with practical tips. Basic knowledge of HPLC is an advantage.


At the end of the course, participants will receive a Springer certificate confirming their participation in the course.


€ 540,- (without VAT) | € 642,60 (inkl. 19% VAT).

Next start dates:

Mondays, 04.11.2024, 11.11.2024 and 18.11.2024, from 02:00 p.m. to 05:00 p.m. (CET). Registration deadline: 20.10.2024. Minimum number of participants: 5

Contact us

For further information about course content, company terms or our in-house seminars please get in touch with us via email or phone. We look forward to hearing from you!

+49 (0)6221-487 8903


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