LC-MS coupling and its applications

Course combines theory, practice and expert tips

The LC-MS technique is a widespread and common coupling technique. The coupling of separation methods such as RPLC and HILIC with (tandem) mass spectrometry is particularly popular. What you can use and how you use the technology is important to know and can be learned!

Springer Campus Certificate courses

  • Continuing education at top level

  • Different learning formats: From self-study to online learning to Attendance courses
  • Incl. consulting and personal support

Course content and process

Introduction to the LC-MS coupling technique

  • Basics of HPLC (RPLC and HILIC)
  • Coupling techniques and ionization: ESI, APCI, APPI
  • Explanation of different MS device types and their use

LC-MS in practice

  • Introduction to method development
  • Selection of solvents and additives (acids, bases, salts)
  • Basics of mass spectrometry (isotope patterns, resolution, mass accuracy, sensitivity)
  • Measurement and calculation of resolution, mass accuracy, S/N and sensitivity
  • Sensitivity test, calibration
  • Performance test, (re)calibration and maintenance
  • LC-MS troubleshooting 

Application examples and evaluation

  • Comparison of ESI and APCI
  • Introductory evaluation of spectra and isotope patterns; nitrogen rule
  • Molecular ions, adduct formation, multiple charges
  • Fragmentation, MS/MS, MSn
  • MS(/MS) measurement modes and explanations (EIC, SIM, MRM, sMRM)
  • Quantification and Matrix Effects
  • Multi-component analysis and NTS

Your top speaker:

PD Dr. habil. Thomas Letzel, born in 1970, studied chemistry (1992-1998) at the Technical University of Munich and the LMU Munich and received his doctorate in 2001 with an environmental analysis topic at the Technical University of Munich and then completed a two-year postdoctoral stay at the Vrijen Universiteit Amsterdam. In 2009 he habilitated at the Technical University of Munich, where he has been teaching and researching as a private lecturer ever since. Until 2018 he was head of the analytical research group AFG at the TUM, 2014-2017 acting head of the professorship for pharmaceutical analysis at the LMU and has been the founder and managing director of the Analytical Research Institute for Non-Target Screening GmbH (AFIN-TS) since 2018. To date, he has developed many new analytical platforms, workflows and strategies for the detection, identification and determination of functional properties of organic molecules from complex mixtures in the various scientific disciplines. The results are reflected in his more than 75 scientific publications and 4 books to date.

All information at a glance:

Course format


Cooperation partner:

Analytisches Forschungsinstitut für Non-Target Screening GmbH

Course duration:

3 days for 3 hours in the afternoon

Target audience: 

The course is aimed at HPLC users who use LC-MS analysis in their laboratory or who want to get started with this technology. The basics and peculiarities of this technique are covered as well as the determination of quantitative results. The aim of this seminar is to impart basic knowledge of LC-MS and to make it easier to get started with this technique with practical tips. Basic knowledge of HPLC is an advantage.


At the end of the course, participants will receive a Springer certificate confirming their participation in the course.


€ 540,- (without VAT) | € 642,60 (inkl. 19% VAT).

Next start dates:

Monday, 09/16/2024, 09/23/2024 and 09/30/2024, from 02:00 p.m. to 05:00 p.m. (CET). Registration deadline: 09/01/2024. 

Minimum number of participants: 5

Contact us

For further information about course content, company terms or our in-house seminars please get in touch with us via email or phone. We look forward to hearing from you!

+49 (0)6221-487 8903

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